The arboretum in on Zoo Rd off of Lake Shore Blvd in Irondequoit.
From the South: Take I-390 North to I-590 N at Exit 15. Follow I-590 N around Rochester and north toward Sea Breeze. On I-590 after the intersection with Rt 104 (where you may also see nomenclature of I-590 as Sea Breeze Dr) there are four 15MPH traffic circles (aka roundabouts/turnabouts). (15MPH, means you have to SLOW DOWN before entering the circles!) Continue on Rt 590 around the first three traffic circles and then go ¾ of the way around the fourth traffic circle and exit onto Durand Blvd. Continue on that street for one mile and turn left onto Zoo Rd (Zoo Rd is one way). The meeting location is at the Kiosk 0.2miles up Zoo Rd on the right (Kiosk is at the second parking area [the first parking area is on the left]). Note: According to Google Maps, Durand Blvd becomes Sweet Fern Rd and then Pine Valley Rd before becoming Lake Shore Blvd at Zoo Rd but you probably will not notice the street name changes. There is a permanent Durand Eastman Arboretum sign and a temporary Sunday Park Hike sign at Zoo Rd. Please watch for bicycles when turning left onto Zoo Rd from Lakeshore Blvd.
From Charlotte and Greece: Take Lake Ontario State Parkway east to Charlotte. At the end of the Parkway, continue straight onto Pattonwood Dr. At the end of Pattonwood, turn right onto St Paul Blvd and then the first left onto Lake Shore Blvd. After 2.4 miles, turn right onto Zoo Rd (Zoo Rd is one way). The meeting location is at the Kiosk 0.2miles up Zoo Rd on the right (Kiosk is at the second parking area [the first parking area is on the left]).
From Canandaigua, Victor and Fairport: Take I-490 W. At Exit 21, follow the 590 N signs. Continue with the directions above From the South.